Cells on Porous Substrates In Vitro and In Vivo

[+] Author and Article Information
K. D. Ansevin, H. G. Welgus

Department of Biology, Rice University, Houston, Texas

C. A. Homsy

Fondren Orthopedic Center, The Methodist Hospital and Baylor College of Medicine, Houston, Texas

B. V. Lipps

Department of Chemical Engineering, Rice University, Houston, Texas

J. Eng. Mater. Technol 96(2), 131-137 (Apr 01, 1974) (7 pages) doi:10.1115/1.3443195 History: Received July 26, 1973; Online August 17, 2010


Disaggregated cells of kidney, lung and liver of 14-day-old chick embryos were cultivated in vitro on several open-pore, organic-polymer sponges and on one gelatin sponge; in some cases this was followed by short-term, subcutaneous implantation into rats. In order to study invasion of connective tissue cells into the porous matrix, the same organic-polymer materials were implanted into rats, and in one experimental series this was followed by in vitro cultivation after they had been retrieved from the animals. The results showed that: 1) epithelial cells have different requirements for invasion and histogenesis from those of connective tissue cells; 2) the type of porous substrate influences morphology of connective tissue that forms within it.

Copyright © 1974 by ASME
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